Sedgewick Rafter Counting Chamber S50
R1,499.00 Excl. VAT
Sedgewick Rafter Counting Chamber in Plastic. Includes 1 cover glass.
- Additional information
- Chamber dimensions
- How to use
An economically priced cell, for one time (or possibly several time) use and for educational/training exercises.
|15 × 15 × 15 cm
50mm x 20mm x 1mm
The cell can be used with either living or preserved material. To fill the cell, place the cover glass across the chamber top. This
allows the air bubbles to escape during the filling procedure. The sample is then taken into a 1ml wide mouthed pipette and then
carefully transferred to the chamber.
Do not overfill the chamber, because the volume of the sample in the chamber must be known exactly and the cover glass must not
float free, but held onto the cell walls by surface tension. During counting, water may evaporate from the chamber. To prevent gas
bubble formation, a small drop of distilled water may be placed on the slide outside the cell, just touching the cell wall and cover
glass. Before the cell count is made the Sedgewick-Rafter chamber should be allowed to stand for at least 15 minutes to allow algae,
or other particles, to settle to the bottom.
The Grid Pattern in the base of the chamber assists the counting and calculation process, by clearly defining a known sample volume
in 1μl blocks Counting in strips is easier, no need to use reticle grids or know the precise area of your field of view.
This makes Pyser Optics’s S50 & S52 Gridded Sedgewick-Rafter Cell perfect for use with a Stereo Zoom Microscope without the
need to carefully set the precise zoom/magnification before counting the sample.
For precise sample preparation and calculation methods, you should follow your own internal or published procedures