Singlepath® Salmonella

Description

Singlepath® Salmonella

Singlepath® Salmonella is a rapid diagnostic test designed to detect Salmonella bacteria in various food samples. Salmonella is a genus of bacteria that can cause human infections, leading to symptoms such as diarrhea, fever, and abdominal cramps. The Singlepath® Salmonella is an immunochromatographic rapid test based on gold-labeled antibodies to detect the presence of a specific antigen, in this case, the Salmonella bacteria. The test includes a nitrocellulose membrane strip that is coated with antibodies specific to Salmonella antigens. When the sample is applied to the test strip, any Salmonella antigens present will bind to the antibodies on the strip, resulting in the development of a visible test line. The test takes approximately 20 minutes to complete, and the results are interpreted based on the presence or absence of the test line. The detection limit can be regarded as less than one colony-forming unit in a 25 g food sample, depending on the serogroup. No additional equipment expenditure is needed.

Benefits

• Reliable: As sensitive as the official culture media method. Provides accurate results:
  -Sensitivity 100% (milk), specificity 100% (milk).
• Fast Result in just 20 minutes.
• Ease-of-use: One-step format avoids working errors during handling.
• Convenient: Simply add a sample and read off the result.
• Safe: Clear and distinct positive or negative test results with a built-in positive control.
• Economical: Rapid results save labour and inventory costs and reduce labour-intensive plating methods. No capital investment is required, for example, for instrumentation such as automated systems.

Application

SINGLEPATH® Salmonella is an immunochromatographic rapid test intended for use in microbiology laboratories analyzing food and animal feeds for the presumptive qualitative detection of Salmonella spp. from food matrices such as meats (raw ground beef and raw ground turkey), spices (black pepper), dairy (dried skimmed milk), dried foods (coconut) and seafood (cooked, peeled frozen prawns).

Specifications

• Agency: according to AOAC® PTM
• Quality Level: 200
• Product Line: Singlepath®
• Shelf Life: 15 mo.
• Packaging: pack of 25 strips
• Technique(s): lateral flow assay: suitable; microbe id | immunological: suitable
• Input: food(s)
• Application(s): food and beverages; pathogen testing
• Storage Temperature: 2–8°C
• Suitability: Citrobacter spp., Salmonella spp.

Additional information

Click here to view Singlepath® Salmonella Datasheet

Salmonella spp. is one of the most common causes of food poisoning worldwide. With an estimated volume of 32 million tests a year, it is probably the food pathogen most frequently tested for. Salmonella spp. have been isolated from most types of raw food (meats, eggs, as well as plant products), and their high resistance to drying combined with a very high heat resistance once dried, makes Salmonella a potential problem in most foods, particularly in dry and semidry products.

Currently, the most commonly used techniques to test for Salmonella in food products are traditional methods based on culture media. Reference methods for the isolation and identification of Salmonella are published by national (AFNOR, AOAC, DIN, FDA-BAM, USDA, etc.) and international (ISO) committees and organisations. Screening for the presence of Salmonella in food products by conventional reference methods is time-consuming and labour-intensive. It usually involves a 3-step technique: non-selective pre-enrichment (18-24h), selective enrichment in two different selective broth media (24-48h) followed by plating on two different selective/indicative agars (24-48h). The whole procedure takes a total of up to 5 days to get a simple yes/no result. For products where a positive release system is important, this means a delay of 5 days before those products can be released into the market.

The requirement of food manufacturers for quicker release of finished products and for cost savings is calling for a change in these methods. Rapid methods, especially highly convenient tests like immunochromatographic one-step devices, are therefore increasingly interesting to food manufacturers and distributors. The general expectation for a rapid test is to be sufficiently sensitive and specific, user-friendly, and cost-effective. Although DNA probes are more specific than immunological tests, they do not always fulfill other relevant user criteria. Immunological tests are therefore often the preferred choice among users of rapid tests.

The new Singlepath® Salmonella immunochromatographic test offers all these benefits and makes testing for Salmonella fast, reliable, and convenient.